Immunostaining:Article Title: CIN85 and CD2AP Are Novel Constituents of Dynamic Tubular Recycling Endosomes That Regulate Recycling Upon Recruitment by MICAL-L1.
Article Snippet: .. Antibody Dilution Source rabbit anti- CD98 1:200 (immunostaining) C817882, LSBio mouse anti- CD98 1:20 (immunostaining) Clone MEM- 108, 315 602, BioLegend mouse anti- GAPDH- HRP 1:1000 (immunoblotting) 60004, ProteinTech rabbit anti- RAB10 1:30 (immunostaining) Clone MJ23, 237703, Abcam mouse anti- MICAL- L1 1:500 (immunostaining) H00085377- B01P, Abnova rabbit anti- MICAL- L1 1:70 (immunostaining) 70017, Novus rabbit anti- MICAL- L1 1:100 (immunoblotting) 1794, LifeTein rabbit anti- EHD1 1:200 (immunoblotting) 1795, LifeTein mouse anti- CIN85 1:20 (immunostaining), 1:200 (immunoblotting) 166862, SantaCruz rabbit anti- CIN85 1:400 (immunoblotting) 121321AP, ProteinTech rabbit anti- CD2AP 1:200 (immunostaining), 1:1000 (immunoblotting) 510461AP, ProteinTech mouse anti- CD2AP 1:50 (immunostaining), 1:300 (immunoblotting) 25272, Santa Cruz mouse anti- cortactin (p80/85) 1:200 (immunostaining) Clone 4F11, 05180I, Sigma mouse anti- capping protein β2 1:300 (immunoblotting) Clone 3F2.3, DSHB Alexa Fluor 568 conjugated goat anti- mouse 1:500 (immunostaining) A11031, Molecular Probes Alexa Fluor 488 conjugated goat anti- mouse 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 568 conjugated goat anti- rabbit 1:500 (immunostaining) A11036, Molecular Probes Alexa Fluor 488 conjugated goat anti- rabbit 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 647 conjugated goat anti- rabbit 1:500 (immunostaining) A21245, Molecular Probes TABLE 2 | Source, sequence, and concentration of siRNA oligonucleotides used. siRNA Source siRNA oligo sequence (5′- 3′) Final concentration (nmol) CIN85 24757, Sigma or L- 014748- 00- 0020, Dharmacon (SmartPool) GAAGUUACCUGCAACUACA (Sigma) 500 (Sigma, HeLa), 600 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) CD2AP 343664, Sigma or L- 012799- 00- 0005, Dharmacon (SmartPool) GACUGUUUCCCUCAAAUUU (Sigma) 360 (Sigma, HeLa), 700 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) MICAL- L1 361239, Sigma or 87469, Sigma GUGAUUCAGCCCUGCCCAA or, GACAAUGUCUUCGAGAAUA 300 EHD1 71015, Sigma CCAAUGUCUUUGGUAAAGA 700 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/tra.70015 by E gyptian N ational Sti. ..
Western Blot:Article Title: CIN85 and CD2AP Are Novel Constituents of Dynamic Tubular Recycling Endosomes That Regulate Recycling Upon Recruitment by MICAL-L1.
Article Snippet: .. Antibody Dilution Source rabbit anti- CD98 1:200 (immunostaining) C817882, LSBio mouse anti- CD98 1:20 (immunostaining) Clone MEM- 108, 315 602, BioLegend mouse anti- GAPDH- HRP 1:1000 (immunoblotting) 60004, ProteinTech rabbit anti- RAB10 1:30 (immunostaining) Clone MJ23, 237703, Abcam mouse anti- MICAL- L1 1:500 (immunostaining) H00085377- B01P, Abnova rabbit anti- MICAL- L1 1:70 (immunostaining) 70017, Novus rabbit anti- MICAL- L1 1:100 (immunoblotting) 1794, LifeTein rabbit anti- EHD1 1:200 (immunoblotting) 1795, LifeTein mouse anti- CIN85 1:20 (immunostaining), 1:200 (immunoblotting) 166862, SantaCruz rabbit anti- CIN85 1:400 (immunoblotting) 121321AP, ProteinTech rabbit anti- CD2AP 1:200 (immunostaining), 1:1000 (immunoblotting) 510461AP, ProteinTech mouse anti- CD2AP 1:50 (immunostaining), 1:300 (immunoblotting) 25272, Santa Cruz mouse anti- cortactin (p80/85) 1:200 (immunostaining) Clone 4F11, 05180I, Sigma mouse anti- capping protein β2 1:300 (immunoblotting) Clone 3F2.3, DSHB Alexa Fluor 568 conjugated goat anti- mouse 1:500 (immunostaining) A11031, Molecular Probes Alexa Fluor 488 conjugated goat anti- mouse 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 568 conjugated goat anti- rabbit 1:500 (immunostaining) A11036, Molecular Probes Alexa Fluor 488 conjugated goat anti- rabbit 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 647 conjugated goat anti- rabbit 1:500 (immunostaining) A21245, Molecular Probes TABLE 2 | Source, sequence, and concentration of siRNA oligonucleotides used. siRNA Source siRNA oligo sequence (5′- 3′) Final concentration (nmol) CIN85 24757, Sigma or L- 014748- 00- 0020, Dharmacon (SmartPool) GAAGUUACCUGCAACUACA (Sigma) 500 (Sigma, HeLa), 600 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) CD2AP 343664, Sigma or L- 012799- 00- 0005, Dharmacon (SmartPool) GACUGUUUCCCUCAAAUUU (Sigma) 360 (Sigma, HeLa), 700 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) MICAL- L1 361239, Sigma or 87469, Sigma GUGAUUCAGCCCUGCCCAA or, GACAAUGUCUUCGAGAAUA 300 EHD1 71015, Sigma CCAAUGUCUUUGGUAAAGA 700 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/tra.70015 by E gyptian N ational Sti. ..
Sequencing:Article Title: CIN85 and CD2AP Are Novel Constituents of Dynamic Tubular Recycling Endosomes That Regulate Recycling Upon Recruitment by MICAL-L1.
Article Snippet: .. Antibody Dilution Source rabbit anti- CD98 1:200 (immunostaining) C817882, LSBio mouse anti- CD98 1:20 (immunostaining) Clone MEM- 108, 315 602, BioLegend mouse anti- GAPDH- HRP 1:1000 (immunoblotting) 60004, ProteinTech rabbit anti- RAB10 1:30 (immunostaining) Clone MJ23, 237703, Abcam mouse anti- MICAL- L1 1:500 (immunostaining) H00085377- B01P, Abnova rabbit anti- MICAL- L1 1:70 (immunostaining) 70017, Novus rabbit anti- MICAL- L1 1:100 (immunoblotting) 1794, LifeTein rabbit anti- EHD1 1:200 (immunoblotting) 1795, LifeTein mouse anti- CIN85 1:20 (immunostaining), 1:200 (immunoblotting) 166862, SantaCruz rabbit anti- CIN85 1:400 (immunoblotting) 121321AP, ProteinTech rabbit anti- CD2AP 1:200 (immunostaining), 1:1000 (immunoblotting) 510461AP, ProteinTech mouse anti- CD2AP 1:50 (immunostaining), 1:300 (immunoblotting) 25272, Santa Cruz mouse anti- cortactin (p80/85) 1:200 (immunostaining) Clone 4F11, 05180I, Sigma mouse anti- capping protein β2 1:300 (immunoblotting) Clone 3F2.3, DSHB Alexa Fluor 568 conjugated goat anti- mouse 1:500 (immunostaining) A11031, Molecular Probes Alexa Fluor 488 conjugated goat anti- mouse 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 568 conjugated goat anti- rabbit 1:500 (immunostaining) A11036, Molecular Probes Alexa Fluor 488 conjugated goat anti- rabbit 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 647 conjugated goat anti- rabbit 1:500 (immunostaining) A21245, Molecular Probes TABLE 2 | Source, sequence, and concentration of siRNA oligonucleotides used. siRNA Source siRNA oligo sequence (5′- 3′) Final concentration (nmol) CIN85 24757, Sigma or L- 014748- 00- 0020, Dharmacon (SmartPool) GAAGUUACCUGCAACUACA (Sigma) 500 (Sigma, HeLa), 600 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) CD2AP 343664, Sigma or L- 012799- 00- 0005, Dharmacon (SmartPool) GACUGUUUCCCUCAAAUUU (Sigma) 360 (Sigma, HeLa), 700 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) MICAL- L1 361239, Sigma or 87469, Sigma GUGAUUCAGCCCUGCCCAA or, GACAAUGUCUUCGAGAAUA 300 EHD1 71015, Sigma CCAAUGUCUUUGGUAAAGA 700 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/tra.70015 by E gyptian N ational Sti. ..
Concentration Assay:Article Title: CIN85 and CD2AP Are Novel Constituents of Dynamic Tubular Recycling Endosomes That Regulate Recycling Upon Recruitment by MICAL-L1.
Article Snippet: .. Antibody Dilution Source rabbit anti- CD98 1:200 (immunostaining) C817882, LSBio mouse anti- CD98 1:20 (immunostaining) Clone MEM- 108, 315 602, BioLegend mouse anti- GAPDH- HRP 1:1000 (immunoblotting) 60004, ProteinTech rabbit anti- RAB10 1:30 (immunostaining) Clone MJ23, 237703, Abcam mouse anti- MICAL- L1 1:500 (immunostaining) H00085377- B01P, Abnova rabbit anti- MICAL- L1 1:70 (immunostaining) 70017, Novus rabbit anti- MICAL- L1 1:100 (immunoblotting) 1794, LifeTein rabbit anti- EHD1 1:200 (immunoblotting) 1795, LifeTein mouse anti- CIN85 1:20 (immunostaining), 1:200 (immunoblotting) 166862, SantaCruz rabbit anti- CIN85 1:400 (immunoblotting) 121321AP, ProteinTech rabbit anti- CD2AP 1:200 (immunostaining), 1:1000 (immunoblotting) 510461AP, ProteinTech mouse anti- CD2AP 1:50 (immunostaining), 1:300 (immunoblotting) 25272, Santa Cruz mouse anti- cortactin (p80/85) 1:200 (immunostaining) Clone 4F11, 05180I, Sigma mouse anti- capping protein β2 1:300 (immunoblotting) Clone 3F2.3, DSHB Alexa Fluor 568 conjugated goat anti- mouse 1:500 (immunostaining) A11031, Molecular Probes Alexa Fluor 488 conjugated goat anti- mouse 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 568 conjugated goat anti- rabbit 1:500 (immunostaining) A11036, Molecular Probes Alexa Fluor 488 conjugated goat anti- rabbit 1:500 (immunostaining) A11029, Molecular Probes Alexa Fluor 647 conjugated goat anti- rabbit 1:500 (immunostaining) A21245, Molecular Probes TABLE 2 | Source, sequence, and concentration of siRNA oligonucleotides used. siRNA Source siRNA oligo sequence (5′- 3′) Final concentration (nmol) CIN85 24757, Sigma or L- 014748- 00- 0020, Dharmacon (SmartPool) GAAGUUACCUGCAACUACA (Sigma) 500 (Sigma, HeLa), 600 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) CD2AP 343664, Sigma or L- 012799- 00- 0005, Dharmacon (SmartPool) GACUGUUUCCCUCAAAUUU (Sigma) 360 (Sigma, HeLa), 700 (Sigma, NSCLC) or 200 (Dharmacon, NSCLC) MICAL- L1 361239, Sigma or 87469, Sigma GUGAUUCAGCCCUGCCCAA or, GACAAUGUCUUCGAGAAUA 300 EHD1 71015, Sigma CCAAUGUCUUUGGUAAAGA 700 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/tra.70015 by E gyptian N ational Sti. ..
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